Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus, a surrogate for SARS-CoV-2 from untreated wastewater

This is a very important and timely paper on the pathway to widespread monitoring of SARS-CoV-2 RNA is in sewage for the purpose of wastewater-based epidemiology. It’s increasingly difficult to keep track of all of the papers that have been put forward, either as published journal papers or (non-peer reviewed) preprints, on this topic. ThoughContinue reading “Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus, a surrogate for SARS-CoV-2 from untreated wastewater”

OPINION: Faecal shedding of SARS-CoV-2, a snapshot of current data and implications for the water industry

There has been quite some talk about SARS-CoV-2 shedding in faeces and what that might mean for the water industry.  As I see it, there are two aspects to this conversation: the first is a concern that sewage may contain infectious SARS-CoV-2 viruses; and the second relates to the more theoretical potential of using SARS-CoV-2Continue reading “OPINION: Faecal shedding of SARS-CoV-2, a snapshot of current data and implications for the water industry”

Presence of SARS-Coronavirus-2 in sewage

Authors: Gertjan Medema and colleagues at KWR in The Netherlands In a non peer reviewed preprint (preprint pdf) report on their use of rt-PCR to detect RNA from SARS-COV-2 at multiple locations.  Their sampling started in February, before COVID19 cases were detected in The Netherlands, and extended when cases were detected at all sites. TheContinue reading “Presence of SARS-Coronavirus-2 in sewage”

SARS-CoV-2–Positive Sputum and Feces After Conversion of Pharyngeal Samples in Patients With COVID-19

This study was reported as a letter to the scientific journal Annals of Internal Medicine on 30 March 2020. The authors report the outcomes of testing for SARS-CoV-2 RNA in sputum and faecal samples from COVID-19 patients after their apparent recovery, which was indicated by throat swabs no longer testing positive. The key finding wasContinue reading “SARS-CoV-2–Positive Sputum and Feces After Conversion of Pharyngeal Samples in Patients With COVID-19”

Dutch scientists recover SARS-CoV-2 RNA from untreated sewage

The possibility of monitoring the presence of SARS-CoV-2 RNA in sewers has been flagged as an important research opportunity (most recently here). Many people are familiar with the idea of monitoring the presence of illicit drugs in sewage as a means of determining temporal and spatial patterns of drug use in communities. A directly analogousContinue reading “Dutch scientists recover SARS-CoV-2 RNA from untreated sewage”