Survey of rapid development of environmental surveillance methods for SARS-CoV-2 detection in wastewater

Environmental surveillance as a part of wastewater-based epidemiology (WBE) of SARS-CoV-2 can provide an early, cost-effective, unbiased community-level indicator of circulating COVID-19 in a population. The objective of this study was to determine how widely SARS-CoV-2 detection in wastewater is being investigated and what methods are used. A survey was developed and distributed, with resultsContinue reading “Survey of rapid development of environmental surveillance methods for SARS-CoV-2 detection in wastewater”

Reproducibility and sensitivity of 36 methods to quantify the SARS-CoV-2 genetic signal in raw wastewater: findings from an interlaboratory methods evaluation in the U.S.

In response to COVID-19, the international water community rapidly developed methods to quantify the SARS-CoV-2 genetic signal in untreated wastewater. Wastewater surveillance using such methods has the potential to complement clinical testing in assessing community health. This interlaboratory assessment evaluated the reproducibility and sensitivity of 36 standard operating procedures (SOPs), divided into eight method groupsContinue reading “Reproducibility and sensitivity of 36 methods to quantify the SARS-CoV-2 genetic signal in raw wastewater: findings from an interlaboratory methods evaluation in the U.S.”

Critical Review and Research Needs of Ozone Applications Related to Virus Inactivation: Potential Implications for SARS-CoV-2

Ozone disinfection has demonstrated high efficacy against enveloped and non-enveloped viruses, including viruses similar in morphology to SARS-CoV-2. Due to this efficacy, numerous gaseous and aqueous phase ozone applications have emerged to potentially inhibit virus persistence in aerosols, surfaces, and water. This review identifies the exposure requirements for virus inactivation and important safety considerations forContinue reading “Critical Review and Research Needs of Ozone Applications Related to Virus Inactivation: Potential Implications for SARS-CoV-2”

Passive sampling of viruses for wastewater-based epidemiology: a case-study of SARS-CoV-2

The shedding of pathogens by infected humans enables the use of sewage monitoring to conduct wastewater-based epidemiology (WBE). Although most WBE studies use data from large sewage treatment plants, timely data from smaller catchments is needed for any targeted public health action. Traditional sampling methods, like autosamplers or grab sampling, are not conducive to quickContinue reading “Passive sampling of viruses for wastewater-based epidemiology: a case-study of SARS-CoV-2”

Early-pandemic wastewater surveillance of SARS-CoV-2 in Southern Nevada: Methodology, occurrence, and incidence/prevalence considerations

Numerous wastewater surveillance studies subsequently confirmed detection of SARS-CoV-2 RNA in wastewater and wastewater-associated solids/sludge. However, the methods employed in early studies vary widely so it is unclear whether differences in reported concentrations reflect true differences in epidemiological conditions, or are instead driven by methodological artifacts. This study aimed to compare the performance of virusContinue reading “Early-pandemic wastewater surveillance of SARS-CoV-2 in Southern Nevada: Methodology, occurrence, and incidence/prevalence considerations”

Primary concentration – The critical step in implementing the wastewater based epidemiology for the COVID-19 pandemic: A mini-review

“This review provides new insights into the primary concentration methods that have been adopted by the eighteen recently reported COVID-19 wastewater detection studies, along with a brief discussion of the mechanisms of the most commonly used virus concentration methods, including the PEG-based separation, electrostatically charged membrane filtration, and ultrafiltration”: continue reading.

Shedding of SARS-CoV-2 in feces and urine and its potential role in person-to-person transmission and the environment-based spread of COVID-19

The authors’ “critical analysis of the available evidence and potential transmissionroutes suggests that the possibility of fecal/urine-oral/ocular transmissionof SARS-CoV-2 is extremely low to negligible except wheredirect person-to-person contact occurs. This is consistent with themany millions of documented cases of COVID-19 worldwide, and thefact that none of these have implicated feces or fecal contaminated materialas partContinue reading “Shedding of SARS-CoV-2 in feces and urine and its potential role in person-to-person transmission and the environment-based spread of COVID-19”

Sewage analysis as a tool for the COVID-19 pandemic response and management: the urgent need for optimised protocols for SARS-CoV-2 detection and quantification

“This review aims at identifying the main issues for consideration, relating to the development of validated methodological protocols for the virus quantitative analysis in wastewater”. However, connecting environmental monitoring to clinical monitoring, and involvement of Health departments/authorities require further investigation for successful integration of sewage monitoring into pandemic response plan.

Rapid expert consultation on environmental surveillance of SARS-CoV-2 in wastewater: summary report (2020)

This report summarizes the findings of the Rapid expert consultation on environmental surveillance of SARS-CoV-2 in wastewater that was organized by the WHO European Centre for Environment and Health on 23 July 2020 in a virtual format. It aimed to facilitate a rapid exchange of current knowledge, experience and practices among countries that are inContinue reading “Rapid expert consultation on environmental surveillance of SARS-CoV-2 in wastewater: summary report (2020)”

Poop tests stop COVID-19 outbreak at University of Arizona

Benefits of targeted swage sampling under a wastewater based epidemiology strategy – By testing dorm wastewater for the coronavirus, the University of Arizona may have stomped out a potential outbreak before it could spread, The Washington Post reports: https://www.sciencemag.org/news/2020/08/poop-tests-stop-covid-19-outbreak-university-arizona?utm_campaign=news_daily_2020-08-28&et_rid=619717835&et_cid=3463056